Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073912" target="_blank" >RIV/00216224:14740/14:00073912 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/14:00427960

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.12428" target="_blank" >10.1111/tpj.12428</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

Popis výsledku v původním jazyce

Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have beenshown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of theTRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits.

Název v anglickém jazyce

Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

Popis výsledku anglicky

Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have beenshown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of theTRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Plant Journal

ISSN

0960-7412

e-ISSN

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Svazek periodika

77

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

770-781

Kód UT WoS článku

000331848700010

EID výsledku v databázi Scopus

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