When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00074135" target="_blank" >RIV/00216224:14740/14:00074135 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/14:00431583

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.12873" target="_blank" >10.1111/nph.12873</a>

Jazyk výsledku

angličtina

Název v původním jazyce

When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

Popis výsledku v původním jazyce

Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes.

Název v anglickém jazyce

When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

Popis výsledku anglicky

Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

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Svazek periodika

203

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

1096-1108

Kód UT WoS článku

000340286000010

EID výsledku v databázi Scopus

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