Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00078951" target="_blank" >RIV/00216224:14740/14:00078951 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.hindawi.com/journals/bmri/2014/691041/" target="_blank" >http://www.hindawi.com/journals/bmri/2014/691041/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2014/691041" target="_blank" >10.1155/2014/691041</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
Popis výsledku v původním jazyce
Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activatedSchwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43.
Název v anglickém jazyce
Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
Popis výsledku anglicky
Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activatedSchwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FH - Neurologie, neurochirurgie, neurovědy
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
BioMed Research International
ISSN
2314-6133
e-ISSN
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Svazek periodika
2014
Číslo periodika v rámci svazku
April
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
"nestránkováno"
Kód UT WoS článku
000335836400001
EID výsledku v databázi Scopus
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