Diverse effects of naturally occurring base lesions on the structure and stability of the human telomere DNA quadruplex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00081336" target="_blank" >RIV/00216224:14740/15:00081336 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/15:00455955

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0300908415002217" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0300908415002217</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biochi.2015.07.013" target="_blank" >10.1016/j.biochi.2015.07.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diverse effects of naturally occurring base lesions on the structure and stability of the human telomere DNA quadruplex

Popis výsledku v původním jazyce

Various base lesions continuously form in cellular nucleic acids and the unrepaired lesions are promutagenic and procarcinogenic. Though natural base lesions have been extensively studied in double-stranded DNA models, these studies are only less than adecade old for non-canonical DNA models, such as quadruplexes. Here we present a report on the effects of three frequently occurring natural lesions that can form in the TTA loops on the structure of the human telomere quadruplex d[AG(3)(TTAG(3))(3)]. Wecompared the effect of the abasic site and 8-oxoadenine replacing adenine and 5-hydroxymethyluracil substituting for thymine. The results showed that the three lesions impacted the stability and quadruplex folding in markedly different ways. The effectsdepended on the type of lesion and the position in the sequence. Analogous lesions of guanine in the G-tetrads extensively destabilized the quadruplex and the effects depended more on the position than on the type of lesion.

Název v anglickém jazyce

Diverse effects of naturally occurring base lesions on the structure and stability of the human telomere DNA quadruplex

Popis výsledku anglicky

Various base lesions continuously form in cellular nucleic acids and the unrepaired lesions are promutagenic and procarcinogenic. Though natural base lesions have been extensively studied in double-stranded DNA models, these studies are only less than adecade old for non-canonical DNA models, such as quadruplexes. Here we present a report on the effects of three frequently occurring natural lesions that can form in the TTA loops on the structure of the human telomere quadruplex d[AG(3)(TTAG(3))(3)]. Wecompared the effect of the abasic site and 8-oxoadenine replacing adenine and 5-hydroxymethyluracil substituting for thymine. The results showed that the three lesions impacted the stability and quadruplex folding in markedly different ways. The effectsdepended on the type of lesion and the position in the sequence. Analogous lesions of guanine in the G-tetrads extensively destabilized the quadruplex and the effects depended more on the position than on the type of lesion.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimie

ISSN

0300-9084

e-ISSN

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Svazek periodika

118

Číslo periodika v rámci svazku

Nov

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

11

Strana od-do

15-25

Kód UT WoS článku

000365062200003

EID výsledku v databázi Scopus

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