TP53 mutation analysis in chronic lymphocytic leukemia: comparison of different detection methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00082234" target="_blank" >RIV/00216224:14740/15:00082234 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/15:00063171

Výsledek na webu

<a href="http://download.springer.com/static/pdf/851/art%253A10.1007%252Fs13277-014-2971-0.pdf?auth66=1421241751_ae3f1991394a2e909c07bb253d606b7c&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/851/art%253A10.1007%252Fs13277-014-2971-0.pdf?auth66=1421241751_ae3f1991394a2e909c07bb253d606b7c&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13277-014-2971-0" target="_blank" >10.1007/s13277-014-2971-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TP53 mutation analysis in chronic lymphocytic leukemia: comparison of different detection methods

Popis výsledku v původním jazyce

TP53 gene defects represent a strong adverse prog- nostic factor for patient survival and treatment resistance in chronic lymphocytic leukemia (CLL). Although various methods for TP53 mutation analysis have been reported, none of them allow the identification of all occurring sequence variants, and the most suitable methodology is still being discussed. The aim of this study was to determine the limita- tions of commonly used methods for TP53 mutation exami- nation in CLL and propose an optimal approachfor their detection. We examined 182 CLL patients enriched for high- risk cases using denaturing high-performance liquid chroma- tography (DHPLC), functional analysis of separated alleles in yeast (FASAY), and the AmpliChip p53 Research Test in parallel. The presence of T53 gene mutations was also evalu- ated using ultra-deep next generation sequencing (NGS) in 69 patients.

Název v anglickém jazyce

TP53 mutation analysis in chronic lymphocytic leukemia: comparison of different detection methods

Popis výsledku anglicky

TP53 gene defects represent a strong adverse prog- nostic factor for patient survival and treatment resistance in chronic lymphocytic leukemia (CLL). Although various methods for TP53 mutation analysis have been reported, none of them allow the identification of all occurring sequence variants, and the most suitable methodology is still being discussed. The aim of this study was to determine the limita- tions of commonly used methods for TP53 mutation exami- nation in CLL and propose an optimal approachfor their detection. We examined 182 CLL patients enriched for high- risk cases using denaturing high-performance liquid chroma- tography (DHPLC), functional analysis of separated alleles in yeast (FASAY), and the AmpliChip p53 Research Test in parallel. The presence of T53 gene mutations was also evalu- ated using ultra-deep next generation sequencing (NGS) in 69 patients.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Tumor Biology

ISSN

1010-4283

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

3371-3380

Kód UT WoS článku

000355199800029

EID výsledku v databázi Scopus

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