G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00083572" target="_blank" >RIV/00216224:14740/15:00083572 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/15:00456709 RIV/00216305:26620/21:PU143827

Výsledek na webu

<a href="http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87" target="_blank" >http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-015-8768-1" target="_blank" >10.1007/s00216-015-8768-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

Popis výsledku v původním jazyce

Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch.

Název v anglickém jazyce

G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

Popis výsledku anglicky

Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP206%2F12%2F2378" target="_blank" >GAP206/12/2378: Vliv konformace a chemické modifikace na chování oligonukleotidů na elektrodách: příspěvek k vývoji DNA senzorů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical chemistry

ISSN

1618-2642

e-ISSN

—

Svazek periodika

407

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

5817-5826

Kód UT WoS článku

000358136900025

EID výsledku v databázi Scopus

—