Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00083622" target="_blank" >RIV/00216224:14740/15:00083622 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/15:00446267

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/tpj.12839/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/tpj.12839/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.12839" target="_blank" >10.1111/tpj.12839</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome

Popis výsledku v původním jazyce

The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plant telomeric sequences.Here we characterize the telomere of Cestrum elegans, work that was a challenge because of its large genome size and few chromosomes (1C 9.76pg; n=8). Significance Statement We previously found that plants from related genera Cestrum, Vestia and Sessea (Solanaceae) lack known telomeric sequences. Here we characterise the Cestrum elegans telomere and the mechanism of its synthesis. We developed an approach combining NGS of untreated and terminally-truncated genomic DNA. We identified, using the RepeatExplorer bioinformatic pipeline, an unusual telomere repeat (TTTTTTAGGG)(n) synthesized by telomerase. That finding will enable studies on evolutionary divergence of telomeres and telomerase, shedding light on telomere interactions and funct

Název v anglickém jazyce

Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome

Popis výsledku anglicky

The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plant telomeric sequences.Here we characterize the telomere of Cestrum elegans, work that was a challenge because of its large genome size and few chromosomes (1C 9.76pg; n=8). Significance Statement We previously found that plants from related genera Cestrum, Vestia and Sessea (Solanaceae) lack known telomeric sequences. Here we characterise the Cestrum elegans telomere and the mechanism of its synthesis. We developed an approach combining NGS of untreated and terminally-truncated genomic DNA. We identified, using the RepeatExplorer bioinformatic pipeline, an unusual telomere repeat (TTTTTTAGGG)(n) synthesized by telomerase. That finding will enable studies on evolutionary divergence of telomeres and telomerase, shedding light on telomere interactions and funct

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Plant Journal

ISSN

0960-7412

e-ISSN

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Svazek periodika

82

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

644-654

Kód UT WoS článku

000354288500009

EID výsledku v databázi Scopus

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