Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088005" target="_blank" >RIV/00216224:14740/16:00088005 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/nph.14049/abstract;jsessionid=06B6FBE103A10C93E72D3E2472F1E40A.f02t03" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/nph.14049/abstract;jsessionid=06B6FBE103A10C93E72D3E2472F1E40A.f02t03</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.14049" target="_blank" >10.1111/nph.14049</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis

Popis výsledku v původním jazyce

• Redirection of intercellular auxin fluxes via relocalization of the PIN3 and PIN7 auxin efflux carriers has been suggested to be necessary for the root gravitropic response. Cytokinins have also been proposed to play a role in controlling root gravitropism, but conclusive evidence is lacking. • We present a detailed study of the dynamics of root bending early after gravistimulation, which revealed a delayed gravitropic response in transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) and cytokinin signaling mutants. Pro35S:AtCKX lines as well as a cytokinin receptor mutant ahk3 showed aberrations in the auxin response distribution in columella cells consistent with defects in the auxin transport machinery. • Using in vivo real-time imaging of PIN3-GFP and PIN7-GFP in AtCKX3 overexpression and ahk3 backgrounds, we observed wild type-like relocalization of PIN proteins in columella early after gravistimulation, with gravity-induced relocalization of PIN7 faster than that of PIN3.

Název v anglickém jazyce

Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis

Popis výsledku anglicky

• Redirection of intercellular auxin fluxes via relocalization of the PIN3 and PIN7 auxin efflux carriers has been suggested to be necessary for the root gravitropic response. Cytokinins have also been proposed to play a role in controlling root gravitropism, but conclusive evidence is lacking. • We present a detailed study of the dynamics of root bending early after gravistimulation, which revealed a delayed gravitropic response in transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) and cytokinin signaling mutants. Pro35S:AtCKX lines as well as a cytokinin receptor mutant ahk3 showed aberrations in the auxin response distribution in columella cells consistent with defects in the auxin transport machinery. • Using in vivo real-time imaging of PIN3-GFP and PIN7-GFP in AtCKX3 overexpression and ahk3 backgrounds, we observed wild type-like relocalization of PIN proteins in columella early after gravistimulation, with gravity-induced relocalization of PIN7 faster than that of PIN3.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

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Svazek periodika

212

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

497-509

Kód UT WoS článku

000383595700023

EID výsledku v databázi Scopus

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