Whole miRNome Sequencing of Human Epileptic Brain Tissue
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088295" target="_blank" >RIV/00216224:14740/16:00088295 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.rnaconference.com/uploadedFiles/RNA_Conference/Agenda/16/2016-RNA-Conference-Brochure.pdf" target="_blank" >http://www.rnaconference.com/uploadedFiles/RNA_Conference/Agenda/16/2016-RNA-Conference-Brochure.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Whole miRNome Sequencing of Human Epileptic Brain Tissue
Popis výsledku v původním jazyce
Mesial temporal lobe epilepsy (mTLE) is a chronic neurological disorder characterized by recurrent seizures. Accumulating evidence of microRNAs´ (miRNAs) potential involvement in pathogenic mechanisms underlying mTLE is emerging. The function of microRNAs in chronic mTLE is still poorly understood partially due to the lack of a clear cut miRNA expression profile of epileptic foci. In the present study, we performed the whole miRNome profiling analysis of the human brain tissue employing novel approach combining next generation sequencing (NGS) with an advanced qPCR technique, miQPCR. miRNA profiles of the hippocampus of autopsy controls and mTLE patients were compared, and analysis revealed differential miRNA expression of 24 miRNAs consistent with published data as well as several miRNA species never considered to play a role in epilepsy before.
Název v anglickém jazyce
Whole miRNome Sequencing of Human Epileptic Brain Tissue
Popis výsledku anglicky
Mesial temporal lobe epilepsy (mTLE) is a chronic neurological disorder characterized by recurrent seizures. Accumulating evidence of microRNAs´ (miRNAs) potential involvement in pathogenic mechanisms underlying mTLE is emerging. The function of microRNAs in chronic mTLE is still poorly understood partially due to the lack of a clear cut miRNA expression profile of epileptic foci. In the present study, we performed the whole miRNome profiling analysis of the human brain tissue employing novel approach combining next generation sequencing (NGS) with an advanced qPCR technique, miQPCR. miRNA profiles of the hippocampus of autopsy controls and mTLE patients were compared, and analysis revealed differential miRNA expression of 24 miRNAs consistent with published data as well as several miRNA species never considered to play a role in epilepsy before.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
FH - Neurologie, neurochirurgie, neurovědy
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA16-04726S" target="_blank" >GA16-04726S: miRNA a biochemické dráhy v epilepsii</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů