Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088530" target="_blank" >RIV/00216224:14740/16:00088530 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5" target="_blank" >http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molp.2016.08.010" target="_blank" >10.1016/j.molp.2016.08.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Popis výsledku v původním jazyce

Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques,we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five a-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1.

Název v anglickém jazyce

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Popis výsledku anglicky

Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques,we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five a-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Plant

ISSN

1674-2052

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

1504-1519

Kód UT WoS článku

000389594100008

EID výsledku v databázi Scopus

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