Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093849" target="_blank" >RIV/00216224:14740/16:00093849 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0257897215302747" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0257897215302747</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.surfcoat.2015.09.035" target="_blank" >10.1016/j.surfcoat.2015.09.035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions

Popis výsledku v původním jazyce

Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, immobilization of the biorecognition biomolecules onto the sensor surface is always required. Cyclopropylamine pulsed plastha polymerization in radio frequency (RF) capacitive discharges was employed to deposit amine thin films on the gold electrode of quartz crystal microbalance (QCM) biosensors and the monoclonal antibody AL-01, specific to human serum albumin (HSA), was immobilized onto the plasma polymer surface. Two different amine plasma polymers were studied. They had a similar amount of primary amine groups, 13-1.5 at.%, as determined by chemical derivatization but their stability in water was different. The 1st type, deposited at the floating potential and 120 Pa, exhibited 16% thickness loss after 24 h in water whereas the 2nd type deposited at RF electrode and 50 Pa was completely stable (2% thickness loss).

Název v anglickém jazyce

Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions

Popis výsledku anglicky

Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, immobilization of the biorecognition biomolecules onto the sensor surface is always required. Cyclopropylamine pulsed plastha polymerization in radio frequency (RF) capacitive discharges was employed to deposit amine thin films on the gold electrode of quartz crystal microbalance (QCM) biosensors and the monoclonal antibody AL-01, specific to human serum albumin (HSA), was immobilized onto the plasma polymer surface. Two different amine plasma polymers were studied. They had a similar amount of primary amine groups, 13-1.5 at.%, as determined by chemical derivatization but their stability in water was different. The 1st type, deposited at the floating potential and 120 Pa, exhibited 16% thickness loss after 24 h in water whereas the 2nd type deposited at RF electrode and 50 Pa was completely stable (2% thickness loss).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BM - Fyzika pevných látek a magnetismus

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SURFACE & COATINGS TECHNOLOGY

ISSN

0257-8972

e-ISSN

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Svazek periodika

290

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

116-123

Kód UT WoS článku

000374370600019

EID výsledku v databázi Scopus

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