Stable gene replacement in barley by targeted double-strand break induction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093884" target="_blank" >RIV/00216224:14740/16:00093884 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erv537" target="_blank" >https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erv537</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erv537" target="_blank" >10.1093/jxb/erv537</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stable gene replacement in barley by targeted double-strand break induction

Popis výsledku v původním jazyce

Using double-strand break induction in the crop plant barley, standard transformation technology allows gene replacement to occur at frequencies suitable for routine application.Gene targeting is becoming an important tool for precision genome engineering in plants. During gene replacement, a variant of gene targeting, transformed DNA integrates into the genome by homologous recombination (HR) to replace resident sequences. We have analysed gene targeting in barley (Hordeum vulgare) using a model system based on double-strand break (DSB) induction by the meganuclease I-SceI and a transgenic, artificial target locus. In the plants we obtained, the donor construct was inserted at the target locus by homology-directed DNA integration in at least two transformants obtained in a single experiment and was stably inherited as a single Mendelian trait. Both events were produced by one-sided integration.

Název v anglickém jazyce

Stable gene replacement in barley by targeted double-strand break induction

Popis výsledku anglicky

Using double-strand break induction in the crop plant barley, standard transformation technology allows gene replacement to occur at frequencies suitable for routine application.Gene targeting is becoming an important tool for precision genome engineering in plants. During gene replacement, a variant of gene targeting, transformed DNA integrates into the genome by homologous recombination (HR) to replace resident sequences. We have analysed gene targeting in barley (Hordeum vulgare) using a model system based on double-strand break (DSB) induction by the meganuclease I-SceI and a transgenic, artificial target locus. In the plants we obtained, the donor construct was inserted at the target locus by homology-directed DNA integration in at least two transformants obtained in a single experiment and was stably inherited as a single Mendelian trait. Both events were produced by one-sided integration.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0189" target="_blank" >EE2.3.20.0189: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

67

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

1433-1445

Kód UT WoS článku

000371928000021

EID výsledku v databázi Scopus

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