Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093963" target="_blank" >RIV/00216224:14740/16:00093963 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-3142-2_13" target="_blank" >10.1007/978-1-4939-3142-2_13</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

Popis výsledku v původním jazyce

The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.

Název v anglickém jazyce

Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

Popis výsledku anglicky

The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Cell Division - Methods and Protocols

ISBN

9781493931415

Počet stran výsledku

14

Strana od-do

169-182

Počet stran knihy

243

Název nakladatele

Springer

Místo vydání

New York

Kód UT WoS kapitoly

000369085800014