Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00097544" target="_blank" >RIV/00216224:14740/17:00097544 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0264127517306469" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0264127517306469</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.matdes.2017.06.057" target="_blank" >10.1016/j.matdes.2017.06.057</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Popis výsledku v původním jazyce

The plasmamodification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groupswas achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion. (C) 2017 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Popis výsledku anglicky

The plasmamodification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groupswas achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion. (C) 2017 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MATERIALS & DESIGN

ISSN

0264-1275

e-ISSN

—

Svazek periodika

132

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

257-265

Kód UT WoS článku

000408688300028

EID výsledku v databázi Scopus

2-s2.0-85022230180