DYNAMICS OF REST-TRF2 INTERACTIONS AND THE FATE OF NEURAL CANCER CELLS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106746" target="_blank" >RIV/00216224:14740/18:00106746 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DYNAMICS OF REST-TRF2 INTERACTIONS AND THE FATE OF NEURAL CANCER CELLS

Popis výsledku v původním jazyce

Glioblastomas (GBM) are the most frequent brain tumors in adults. No efficient treatment of GMB has been developed so far. The median maximum survival rate of glioblastoma-suffering patients is 12 months. The Repressor Element-1 Silencing Transcription factor (REST), also known as Neuron-Restrictive Silencing Factor (NRSF) is a transcriptional repressor recognized as a negative regulator of many genes, mainly neuronal. REST is usually expressed in nonneuronal tissues and stem cells, wherein it suppresses neuronal differentiation. REST is also present in differentiated neurons during the postnatal brain development and in normal aging, where it promotes neuroprotection by repressing genes involved in oxidative stress. But what makes REST so interesting? REST is crucial for self-renewal of cancer stem cell and brain tumor cells such as GBM. Shelterin protein TRF2 protects REST against proteasomal degradation, facilitates the physiological self-renewal of neural progenitor cells and the pathological uncontrolled proliferation of cancer cells. Identification of the interacting regions and following disruption of TRF2-REST interaction targets REST for proteasomal degradation. This could be the way how we can inhibit cancer stem cells and whole GLM tumor proliferation. In our initial studies we used FLIM-FRET (Fluorescence Lifetime Imaging Microscopy – Fluorescence Resonance Energy Transfer), PLA (proximity ligation assay) and pull-down assay to determine interacting regions of REST and TRF2.

Název v anglickém jazyce

DYNAMICS OF REST-TRF2 INTERACTIONS AND THE FATE OF NEURAL CANCER CELLS

Popis výsledku anglicky

Glioblastomas (GBM) are the most frequent brain tumors in adults. No efficient treatment of GMB has been developed so far. The median maximum survival rate of glioblastoma-suffering patients is 12 months. The Repressor Element-1 Silencing Transcription factor (REST), also known as Neuron-Restrictive Silencing Factor (NRSF) is a transcriptional repressor recognized as a negative regulator of many genes, mainly neuronal. REST is usually expressed in nonneuronal tissues and stem cells, wherein it suppresses neuronal differentiation. REST is also present in differentiated neurons during the postnatal brain development and in normal aging, where it promotes neuroprotection by repressing genes involved in oxidative stress. But what makes REST so interesting? REST is crucial for self-renewal of cancer stem cell and brain tumor cells such as GBM. Shelterin protein TRF2 protects REST against proteasomal degradation, facilitates the physiological self-renewal of neural progenitor cells and the pathological uncontrolled proliferation of cancer cells. Identification of the interacting regions and following disruption of TRF2-REST interaction targets REST for proteasomal degradation. This could be the way how we can inhibit cancer stem cells and whole GLM tumor proliferation. In our initial studies we used FLIM-FRET (Fluorescence Lifetime Imaging Microscopy – Fluorescence Resonance Energy Transfer), PLA (proximity ligation assay) and pull-down assay to determine interacting regions of REST and TRF2.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

15 th International Interdisciplinary Meeting on Bioanalysis

ISBN

9788090495975

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

276-282

Název nakladatele

Institute of Analytical Chemistry of the CAS, v. v. i., Brno, Czech Republic

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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