Study of lectins from opportunistic pathogens and their interaction with normal human cells.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00107308" target="_blank" >RIV/00216224:14740/19:00107308 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of lectins from opportunistic pathogens and their interaction with normal human cells.

Popis výsledku v původním jazyce

Lectins are carbohydrate-binding proteins, highly specific for sugar moieties. They can be involved in the attachment of bacteria and viruses on their host epithelial surfaces, which is one of the most critical step leading to infection. Since the antibiotic strategy is not fully efficient anymore, new ways to cure bacterial infections are under development. One of the possibilities is anti-adhesion/anti-lectin therapy where the lectin can be inhibited by its ligand. For bringing this therapy to the medicine, first we need to identify a binding partner of the lectin and then to provide inhibition tests which approve specificity of lectins. In this work we studied interactions between lectins from Burkholderia cenocepacia and Photorhabdus luminescens, with several different types of normal non-cancerous human cells. Both lectins are fucose specific and calcium independent. The interaction was detected by fluorescence microscopy and for each observed interaction we provided inhibition tests. The lectin from B. cenocepacia interacted with surface glycoproteins of embryonal stem cells and early lung epithelial progenitors but did not bind any ligand on the surface of neural stem cells. In contrary, the lectin from P. luminescens interacted only with mesenchymal stromal cells. The obtained results may bring new promising possibilities to the research and medicine.

Název v anglickém jazyce

Study of lectins from opportunistic pathogens and their interaction with normal human cells.

Popis výsledku anglicky

Lectins are carbohydrate-binding proteins, highly specific for sugar moieties. They can be involved in the attachment of bacteria and viruses on their host epithelial surfaces, which is one of the most critical step leading to infection. Since the antibiotic strategy is not fully efficient anymore, new ways to cure bacterial infections are under development. One of the possibilities is anti-adhesion/anti-lectin therapy where the lectin can be inhibited by its ligand. For bringing this therapy to the medicine, first we need to identify a binding partner of the lectin and then to provide inhibition tests which approve specificity of lectins. In this work we studied interactions between lectins from Burkholderia cenocepacia and Photorhabdus luminescens, with several different types of normal non-cancerous human cells. Both lectins are fucose specific and calcium independent. The interaction was detected by fluorescence microscopy and for each observed interaction we provided inhibition tests. The lectin from B. cenocepacia interacted with surface glycoproteins of embryonal stem cells and early lung epithelial progenitors but did not bind any ligand on the surface of neural stem cells. In contrary, the lectin from P. luminescens interacted only with mesenchymal stromal cells. The obtained results may bring new promising possibilities to the research and medicine.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů