Structure and genome ejection mechanism of Staphylococcus aureus phage P68

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00107744" target="_blank" >RIV/00216224:14740/19:00107744 - isvavai.cz</a>

Výsledek na webu

<a href="https://advances.sciencemag.org/content/5/10/eaaw7414" target="_blank" >https://advances.sciencemag.org/content/5/10/eaaw7414</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/sciadv.aaw7414" target="_blank" >10.1126/sciadv.aaw7414</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure and genome ejection mechanism of Staphylococcus aureus phage P68

Popis výsledku v původním jazyce

Phages infecting Staphylococcus aureus can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native S. aureus phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm.

Název v anglickém jazyce

Structure and genome ejection mechanism of Staphylococcus aureus phage P68

Popis výsledku anglicky

Phages infecting Staphylococcus aureus can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native S. aureus phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science advances

ISSN

2375-2548

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

„eaaw7414“

Kód UT WoS článku

000491132700068

EID výsledku v databázi Scopus

2-s2.0-85073872322