Epigenetic pattern of plant telomeres with non-canonical sequence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00110133" target="_blank" >RIV/00216224:14740/19:00110133 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Epigenetic pattern of plant telomeres with non-canonical sequence

Popis výsledku v původním jazyce

Telomeres are nucleoprotein structures at the ends of linear eukaryotic chromosomes. They are crucial for the maintenance of genome integrity protecting the genetic information inside the chromosomes from the lost due to the incomplete DNA replication, and distinguishing natural chromosome ends from DNA breaks. Telomeres consist of tandemly repeated short units, sequence of which is relatively conserved, e.g., TTAGGG in mammals or TTTAGGG in most plants. Recently, plants with non-canonical telomeres were described, including Genlisea hispidula (TTCAGG/TTTCAGG), Cestrum elegans (TTTTTTAGGG), plants from several families in the Asparagales order (TTAGGG), and Allium species (CTCGGTTATGGG). We analyzed epigenetic properties of telomere histones in plants with non-canonical telomeres (C. elegans, G. hispidula, A. cepa, Narcissus pseudonarcissus) and with canonical telomeres (A. thaliana, N. tabacum, N. benthamiana, Genlisea nigrocaulis). Next, we analyzed telomere chromatin captured from N. benthamiana leaves transiently transformed by telomere CRISPR-dCas9-eGFP, and from A. thaliana stably transformed with TALE_telo C-3xGFP. Chromatin was immunoprecipitated with antibodies against specific histone marks and DNA was hybridized with telomere probe. Despite of differences in telomere lengths and sequences, tested plant species exhibited two kinds of epigenetic patterns of telomere histones. i) Arabidopsis – like pattern (A. thaliana, G. hispidula, G. nigrocaulis, A. cepa, N. pseudonarcissus), with telomere histones decorated predominantly by H3K9me2. ii) tobacco – like pattern (N. tabacum, N. benthamiana, C. elegans), with telomere histones decorated predominantly by H3K27me3. Our data suggests that epigenetic modifications of plant telomere histones are directly related neither to the length of telomeres, nor to their particular sequence.

Název v anglickém jazyce

Epigenetic pattern of plant telomeres with non-canonical sequence

Popis výsledku anglicky

Telomeres are nucleoprotein structures at the ends of linear eukaryotic chromosomes. They are crucial for the maintenance of genome integrity protecting the genetic information inside the chromosomes from the lost due to the incomplete DNA replication, and distinguishing natural chromosome ends from DNA breaks. Telomeres consist of tandemly repeated short units, sequence of which is relatively conserved, e.g., TTAGGG in mammals or TTTAGGG in most plants. Recently, plants with non-canonical telomeres were described, including Genlisea hispidula (TTCAGG/TTTCAGG), Cestrum elegans (TTTTTTAGGG), plants from several families in the Asparagales order (TTAGGG), and Allium species (CTCGGTTATGGG). We analyzed epigenetic properties of telomere histones in plants with non-canonical telomeres (C. elegans, G. hispidula, A. cepa, Narcissus pseudonarcissus) and with canonical telomeres (A. thaliana, N. tabacum, N. benthamiana, Genlisea nigrocaulis). Next, we analyzed telomere chromatin captured from N. benthamiana leaves transiently transformed by telomere CRISPR-dCas9-eGFP, and from A. thaliana stably transformed with TALE_telo C-3xGFP. Chromatin was immunoprecipitated with antibodies against specific histone marks and DNA was hybridized with telomere probe. Despite of differences in telomere lengths and sequences, tested plant species exhibited two kinds of epigenetic patterns of telomere histones. i) Arabidopsis – like pattern (A. thaliana, G. hispidula, G. nigrocaulis, A. cepa, N. pseudonarcissus), with telomere histones decorated predominantly by H3K9me2. ii) tobacco – like pattern (N. tabacum, N. benthamiana, C. elegans), with telomere histones decorated predominantly by H3K27me3. Our data suggests that epigenetic modifications of plant telomere histones are directly related neither to the length of telomeres, nor to their particular sequence.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů