Memory CD4(+) T cells are generated in the human fetal intestine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113268" target="_blank" >RIV/00216224:14740/19:00113268 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s41590-018-0294-9" target="_blank" >http://dx.doi.org/10.1038/s41590-018-0294-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41590-018-0294-9" target="_blank" >10.1038/s41590-018-0294-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Memory CD4(+) T cells are generated in the human fetal intestine

Popis výsledku v původním jazyce

The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO(+) T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4(+) T cell compartment in the human fetal intestine. We identified 22 CD4(+) T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4(+) T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-gamma and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4(+) T cells. Imaging mass cytometry indicated that memory-like CD4(+) T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4(+) T cells in the human fetal intestine that is consistent with exposure to foreign antigens.

Název v anglickém jazyce

Memory CD4(+) T cells are generated in the human fetal intestine

Popis výsledku anglicky

The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO(+) T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4(+) T cell compartment in the human fetal intestine. We identified 22 CD4(+) T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4(+) T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-gamma and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4(+) T cells. Imaging mass cytometry indicated that memory-like CD4(+) T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4(+) T cells in the human fetal intestine that is consistent with exposure to foreign antigens.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature immunology

ISSN

1529-2908

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

301-314

Kód UT WoS článku

000458893600015

EID výsledku v databázi Scopus

2-s2.0-85060343432