Cdk11 is a Novel Player in Non-coding RNA Expression
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118404" target="_blank" >RIV/00216224:14740/20:00118404 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cdk11 is a Novel Player in Non-coding RNA Expression
Popis výsledku v původním jazyce
Cdk11 is a poorly-characterized cyclin-dependent kinase with described functions in transcription of mRNA, alternative splicing and 3’end processing of HIV mRNA. Our aim was to better define its cellular roles via identification of novel interacting partners and candidate substrates combined with data from microarray experiment after knock-down of Cdk11. We utilized BioID method to probe for relevant proteins connected to Cdk11. Microarray data showed differential expression of a subset of non-coding genes. Proteins implicated in control of their expression and processing were identified in BioID screen as candidate interacting partners of Cdk11. We utilized also Cdk11 analog-sensitive mutant (designed using Shokat’s lab approach) to test the candidate interacting partners as substrates of Cdk11 in vitro. Altogether, our data uncover novel role of Cdk11 in the expression of subset of non-coding RNAs
Název v anglickém jazyce
Cdk11 is a Novel Player in Non-coding RNA Expression
Popis výsledku anglicky
Cdk11 is a poorly-characterized cyclin-dependent kinase with described functions in transcription of mRNA, alternative splicing and 3’end processing of HIV mRNA. Our aim was to better define its cellular roles via identification of novel interacting partners and candidate substrates combined with data from microarray experiment after knock-down of Cdk11. We utilized BioID method to probe for relevant proteins connected to Cdk11. Microarray data showed differential expression of a subset of non-coding genes. Proteins implicated in control of their expression and processing were identified in BioID screen as candidate interacting partners of Cdk11. We utilized also Cdk11 analog-sensitive mutant (designed using Shokat’s lab approach) to test the candidate interacting partners as substrates of Cdk11 in vitro. Altogether, our data uncover novel role of Cdk11 in the expression of subset of non-coding RNAs
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů