The genetic and epigenetic landscape of the Arabidopsis centromeres

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00119657" target="_blank" >RIV/00216224:14740/21:00119657 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.science.org/doi/10.1126/science.abi7489" target="_blank" >https://www.science.org/doi/10.1126/science.abi7489</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/science.abi7489" target="_blank" >10.1126/science.abi7489</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The genetic and epigenetic landscape of the Arabidopsis centromeres

Popis výsledku v původním jazyce

Centromeres attach chromosomes to spindle microtubules during cell division and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used long-read sequencing to generate the Col-CEN Arabidopsis thaliana genome assembly that resolves all five centromeres. The centromeres consist of megabase-scale tandemly repeated satellite arrays, which support CENTROMERE SPECIFIC HISTONE H3 (CENH3) occupancy and are densely DNA methylated, with satellite variants private to each chromosome. CENH3 preferentially occupies satellites that show the least amount of divergence and occur in higher-order repeats. The centromeres are invaded by ATHILA retrotransposons, which disrupt genetic and epigenetic organization. Centromeric crossover recombination is suppressed, yet low levels of meiotic DNA double-strand breaks occur that are regulated by DNA methylation. We propose that Arabidopsis centromeres are evolving through cycles of satellite homogenization and retrotransposon-driven diversification.

Název v anglickém jazyce

The genetic and epigenetic landscape of the Arabidopsis centromeres

Popis výsledku anglicky

Centromeres attach chromosomes to spindle microtubules during cell division and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used long-read sequencing to generate the Col-CEN Arabidopsis thaliana genome assembly that resolves all five centromeres. The centromeres consist of megabase-scale tandemly repeated satellite arrays, which support CENTROMERE SPECIFIC HISTONE H3 (CENH3) occupancy and are densely DNA methylated, with satellite variants private to each chromosome. CENH3 preferentially occupies satellites that show the least amount of divergence and occur in higher-order repeats. The centromeres are invaded by ATHILA retrotransposons, which disrupt genetic and epigenetic organization. Centromeric crossover recombination is suppressed, yet low levels of meiotic DNA double-strand breaks occur that are regulated by DNA methylation. We propose that Arabidopsis centromeres are evolving through cycles of satellite homogenization and retrotransposon-driven diversification.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA21-03909S" target="_blank" >GA21-03909S: Odhalení evolučních tajů lničky seté a příbuzných druhů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science

ISSN

0036-8075

e-ISSN

—

Svazek periodika

374

Číslo periodika v rámci svazku

6569

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

88

Strana od-do

„840“-„+“

Kód UT WoS článku

000717915400028

EID výsledku v databázi Scopus

2-s2.0-85118956061