Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00124438" target="_blank" >RIV/00216224:14740/21:00124438 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.embopress.org/doi/full/10.15252/embj.2020106449" target="_blank" >https://www.embopress.org/doi/full/10.15252/embj.2020106449</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embj.2020106449" target="_blank" >10.15252/embj.2020106449</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation
Popis výsledku v původním jazyce
In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the ribosomal A and E sites, its exact mechanism of action is unclear. Here, we show that eEF3 acts at the mRNA-tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slowdown in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of native eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states, as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.
Název v anglickém jazyce
Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation
Popis výsledku anglicky
In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the ribosomal A and E sites, its exact mechanism of action is unclear. Here, we show that eEF3 acts at the mRNA-tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slowdown in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of native eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states, as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
—
Návaznosti
—
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
EMBO Journal
ISSN
0261-4189
e-ISSN
—
Svazek periodika
40
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
20
Strana od-do
1-20
Kód UT WoS článku
000615851800001
EID výsledku v databázi Scopus
2-s2.0-85100560703