Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F22%3A00135974" target="_blank" >RIV/00216224:14740/22:00135974 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-2253-7_8" target="_blank" >10.1007/978-1-0716-2253-7_8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Popis výsledku v původním jazyce

Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition.

Název v anglickém jazyce

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Popis výsledku anglicky

Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EF15_003%2F0000479" target="_blank" >EF15_003/0000479: Regulace rostlinné meiózy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Gametogenesis. Methods in Molecular Biology, vol 2484

ISBN

9781071622520

Počet stran výsledku

13

Strana od-do

93-105

Počet stran knihy

382

Název nakladatele

Humana Press Inc.

Místo vydání

Humana New York, NY

Kód UT WoS kapitoly

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