Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132768" target="_blank" >RIV/00216224:14740/23:00132768 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.embl.org/about/info/course-and-conference-office/events/can23-01/#vf-tabs__section-overview" target="_blank" >https://www.embl.org/about/info/course-and-conference-office/events/can23-01/#vf-tabs__section-overview</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.

Popis výsledku v původním jazyce

Studying chronic lymphocytic leukemia (CLL) in vitro is challenging due to its complexity and dependency of CLL cells on their microenvironment. An integral part of the natural CLL microenvironment is the three-dimensional (3D) spatial organization, which facilitates frequent cell-to-cell and cell-to-matrix contacts. Therefore, we aimed to mimic the natural tissue architecture by implementing a 3D in vitro culture. We hypothesized that compared to conventional culture, the additional dimension and increased cell-to-matrix contacts would enhance the prosurvival stimuli in CLL cells. We cultured CLL cells in two materials: collagen scaffolds, or gel composed of carboxymethyl cellulose and polyethylene glycol (CMC-PEG). We assessed cell distribution, morphology, and viability via microscopy, and measured the metabolic activity by AlamarBlue assay. Gene expression (MYC, VCAM1, MCL1, CXCR4, CCL4) was analyzed using qPCR to understand the effects of novel culture approaches on adhesion, apoptosis, and intercellular interactions of CLL cells co-cultured with bone marrow stromal cells (BMSCs) in 3D.

Název v anglickém jazyce

Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.

Popis výsledku anglicky

Studying chronic lymphocytic leukemia (CLL) in vitro is challenging due to its complexity and dependency of CLL cells on their microenvironment. An integral part of the natural CLL microenvironment is the three-dimensional (3D) spatial organization, which facilitates frequent cell-to-cell and cell-to-matrix contacts. Therefore, we aimed to mimic the natural tissue architecture by implementing a 3D in vitro culture. We hypothesized that compared to conventional culture, the additional dimension and increased cell-to-matrix contacts would enhance the prosurvival stimuli in CLL cells. We cultured CLL cells in two materials: collagen scaffolds, or gel composed of carboxymethyl cellulose and polyethylene glycol (CMC-PEG). We assessed cell distribution, morphology, and viability via microscopy, and measured the metabolic activity by AlamarBlue assay. Gene expression (MYC, VCAM1, MCL1, CXCR4, CCL4) was analyzed using qPCR to understand the effects of novel culture approaches on adhesion, apoptosis, and intercellular interactions of CLL cells co-cultured with bone marrow stromal cells (BMSCs) in 3D.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů