The role of long non-coding RNAs in BCR-mediated CLL activation.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132967" target="_blank" >RIV/00216224:14740/23:00132967 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.ceitec.eu/ceitec-phd-conference-2023/a4489" target="_blank" >https://www.ceitec.eu/ceitec-phd-conference-2023/a4489</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The role of long non-coding RNAs in BCR-mediated CLL activation.
Popis výsledku v původním jazyce
B cell Receptor (BCR) plays a pivotal role in providing maturation and survival signals for B cells. However, dysregulation of the BCR pathway is a fundamental characteristic observed in numerous B cell malignancies, including chronic lymphocytic leukemia (CLL), revealing its importance in disease progression. Despite the absence of recurrent mutations found in the BCR-related genes of untreated cases, BCR inhibitors have shown a universal clinical response in CLL patients. We and others have shown that short non-coding RNAs, namely microRNAs, can (dys)regulate the BCR signaling propensity, but it is still unclear if long non-coding RNAs (lncRNAs) play a role in BCRactivation. Hence, we hypothesized that lncRNAs could be involved in BCR-mediated CLL activation. To address our hypothesis, we performed differential lncRNA expression analysis in CLL cells from patients treated with BCR inhibitors and cross-validated in CLL intraclonal subpopulations with high BCR activity (CXCR4dim CD5bright) vs. low BCR activity (CXCR4bright CD5dim). We found 12 lncRNAs related to the BCR pathway inhibition/activity. Out of these lncRNAs, we selected a lncRNA that belongs to a class of lncRNA called long intergenic non-coding RNA (lincRNAs) which often play a role in trans-activating signaling pathways.
Název v anglickém jazyce
The role of long non-coding RNAs in BCR-mediated CLL activation.
Popis výsledku anglicky
B cell Receptor (BCR) plays a pivotal role in providing maturation and survival signals for B cells. However, dysregulation of the BCR pathway is a fundamental characteristic observed in numerous B cell malignancies, including chronic lymphocytic leukemia (CLL), revealing its importance in disease progression. Despite the absence of recurrent mutations found in the BCR-related genes of untreated cases, BCR inhibitors have shown a universal clinical response in CLL patients. We and others have shown that short non-coding RNAs, namely microRNAs, can (dys)regulate the BCR signaling propensity, but it is still unclear if long non-coding RNAs (lncRNAs) play a role in BCRactivation. Hence, we hypothesized that lncRNAs could be involved in BCR-mediated CLL activation. To address our hypothesis, we performed differential lncRNA expression analysis in CLL cells from patients treated with BCR inhibitors and cross-validated in CLL intraclonal subpopulations with high BCR activity (CXCR4dim CD5bright) vs. low BCR activity (CXCR4bright CD5dim). We found 12 lncRNAs related to the BCR pathway inhibition/activity. Out of these lncRNAs, we selected a lncRNA that belongs to a class of lncRNA called long intergenic non-coding RNA (lincRNAs) which often play a role in trans-activating signaling pathways.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů