Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132976" target="_blank" >RIV/00216224:14740/23:00132976 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.ceitec.eu/ceitec-phd-conference-2023/a4489" target="_blank" >https://www.ceitec.eu/ceitec-phd-conference-2023/a4489</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
Popis výsledku v původním jazyce
Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.
Název v anglickém jazyce
Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
Popis výsledku anglicky
Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů