Unraveling the process of thermoregulation during the seed development in Brassica napus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00138955" target="_blank" >RIV/00216224:14740/24:00138955 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Unraveling the process of thermoregulation during the seed development in Brassica napus

Popis výsledku v původním jazyce

We observed that high temperatures above the optimum growth temperature resulted in the production of seeds with ruptured seed coats in Brassica napus cv Topas. This phenotype is associated with accelerated embryo development. However, the relationships between the three events – high temperature, embryo growth pace, and seed coat rupture - remain unclear. To investigate the occurrence of temperature-induced seed coat rupture, we combined detailed phenotyping approaches of oilseed rape seeds with transcriptomics, histology, immunolabelling, hormone and cell wall profiling. Our data suggest that high temperatures accelerate embryo growth, resulting in larger embryos but not larger seeds. Such large embryos exert a putative mechanical pressure on the seed coat cells, for which we observed a reduced cell layer thickness. The seed coat began to mature prematurely with the accumulation of demethylesterified pectin, possibly making the cell wall stiffer, which eventually ruptured. Our data present novel observations on the impact of high temperatures on seed development, tackling issues linked to Seed biomechanic features.

Název v anglickém jazyce

Unraveling the process of thermoregulation during the seed development in Brassica napus

Popis výsledku anglicky

We observed that high temperatures above the optimum growth temperature resulted in the production of seeds with ruptured seed coats in Brassica napus cv Topas. This phenotype is associated with accelerated embryo development. However, the relationships between the three events – high temperature, embryo growth pace, and seed coat rupture - remain unclear. To investigate the occurrence of temperature-induced seed coat rupture, we combined detailed phenotyping approaches of oilseed rape seeds with transcriptomics, histology, immunolabelling, hormone and cell wall profiling. Our data suggest that high temperatures accelerate embryo growth, resulting in larger embryos but not larger seeds. Such large embryos exert a putative mechanical pressure on the seed coat cells, for which we observed a reduced cell layer thickness. The seed coat began to mature prematurely with the accumulation of demethylesterified pectin, possibly making the cell wall stiffer, which eventually ruptured. Our data present novel observations on the impact of high temperatures on seed development, tackling issues linked to Seed biomechanic features.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: Nové poznatky pro plodiny nové generace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů