Unraveling the genetic and epigenetic impacts of large-scale chromosomal rearrangements in Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00138962" target="_blank" >RIV/00216224:14740/24:00138962 - isvavai.cz</a>

Výsledek na webu

<a href="https://meetings.embo.org/event/24-telomere" target="_blank" >https://meetings.embo.org/event/24-telomere</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Unraveling the genetic and epigenetic impacts of large-scale chromosomal rearrangements in Arabidopsis thaliana

Popis výsledku v původním jazyce

Chromosomal rearrangements can result from large-scale structural changes in the genome, such as inversions, translocations, duplications, and deletions. These rearrangements often have significant implications for the plant's genetic makeup and epigenetic regulation. Importantly, it is even possible to simulate large-scale genome re-arrangements mimicking those that occurred naturally during plant evolution. Using CRISPR/Cas9 approach, Arabidopsis thaliana lines with stable reciprocal translocations of chromosome arms (Beying et al., 2020) and inverted chromosome (Rönspies, 2022) were constructed. We studied phenotype, telomere lengths, levels of transcripts and chromatin structure in homozygous generations of these plants. These analyses are supposed to reveal whether and how chromatin organization is affected in response to changes in genome organization, and whether and how respective changes are inter-related. Understanding the impact of chromosomal rearrangements in Arabidopsis thaliana can provide valuable insights into the evolutionary dynamics and adaptive potential of plant genomes. Beying, N.; Schmidt, C.; Pacher, M.; Houben, A.; Puchta, H. CRISPR-Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis. Nat Plants 2020, 6, 638-645. Rönspies, M.; Schmidt, C.; Schindele, P.; Lieberman-Lazarovich, M.; Houben, A.; Puchta, H. Massive crossover suppression by CRISPR-Cas-mediated plant chromosome engineering.

Název v anglickém jazyce

Unraveling the genetic and epigenetic impacts of large-scale chromosomal rearrangements in Arabidopsis thaliana

Popis výsledku anglicky

Chromosomal rearrangements can result from large-scale structural changes in the genome, such as inversions, translocations, duplications, and deletions. These rearrangements often have significant implications for the plant's genetic makeup and epigenetic regulation. Importantly, it is even possible to simulate large-scale genome re-arrangements mimicking those that occurred naturally during plant evolution. Using CRISPR/Cas9 approach, Arabidopsis thaliana lines with stable reciprocal translocations of chromosome arms (Beying et al., 2020) and inverted chromosome (Rönspies, 2022) were constructed. We studied phenotype, telomere lengths, levels of transcripts and chromatin structure in homozygous generations of these plants. These analyses are supposed to reveal whether and how chromatin organization is affected in response to changes in genome organization, and whether and how respective changes are inter-related. Understanding the impact of chromosomal rearrangements in Arabidopsis thaliana can provide valuable insights into the evolutionary dynamics and adaptive potential of plant genomes. Beying, N.; Schmidt, C.; Pacher, M.; Houben, A.; Puchta, H. CRISPR-Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis. Nat Plants 2020, 6, 638-645. Rönspies, M.; Schmidt, C.; Schindele, P.; Lieberman-Lazarovich, M.; Houben, A.; Puchta, H. Massive crossover suppression by CRISPR-Cas-mediated plant chromosome engineering.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů