Chapter 16 - Interactions among the three protein states

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00138978" target="_blank" >RIV/00216224:14740/24:00138978 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/book/9780443218095/the-three-functional-states-of-proteins" target="_blank" >https://www.sciencedirect.com/book/9780443218095/the-three-functional-states-of-proteins</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-443-21809-5.00022-3" target="_blank" >10.1016/B978-0-443-21809-5.00022-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chapter 16 - Interactions among the three protein states

Popis výsledku v původním jazyce

Interactions between the three states of proteins (structured, intrinsically disordered, and phase separated) can be probed by a variety of experimental methods. First, we review fundamental physical forces determining structural features and intermolecular interactions of all three states of proteins. We classify individual contributions of the interactions, comment on their theoretical treatment, stress the importance of water as a solvent, and discuss the hierarchy of protein structures. Then we briefly mention methods of structure determination at the atomic resolution and describe in more detail selected methods probing specific features of intra- and intermolecular protein interactions. The selection includes various methods of nuclear magnetic resonance spectroscopy (including analysis of chemical shifts, relaxation effects, and measurements of translational diffusion and proton exchange) and methods reflecting hydrodynamic behavior, namely, analytical ultracentrifugation and small-angle X-ray scattering. The text does not have an ambition to provide actual protocols or a complete list of applications but tries to comment on the use of the methods for the three states of proteins.

Název v anglickém jazyce

Chapter 16 - Interactions among the three protein states

Popis výsledku anglicky

Interactions between the three states of proteins (structured, intrinsically disordered, and phase separated) can be probed by a variety of experimental methods. First, we review fundamental physical forces determining structural features and intermolecular interactions of all three states of proteins. We classify individual contributions of the interactions, comment on their theoretical treatment, stress the importance of water as a solvent, and discuss the hierarchy of protein structures. Then we briefly mention methods of structure determination at the atomic resolution and describe in more detail selected methods probing specific features of intra- and intermolecular protein interactions. The selection includes various methods of nuclear magnetic resonance spectroscopy (including analysis of chemical shifts, relaxation effects, and measurements of translational diffusion and proton exchange) and methods reflecting hydrodynamic behavior, namely, analytical ultracentrifugation and small-angle X-ray scattering. The text does not have an ambition to provide actual protocols or a complete list of applications but tries to comment on the use of the methods for the three states of proteins.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

The Three Functional States of Proteins. Structured, Intrinsically Disordered, and Phase Separated

ISBN

9780443218095

Počet stran výsledku

17

Strana od-do

297-313

Počet stran knihy

456

Název nakladatele

Academic Press

Místo vydání

525 B ST, STE 1900, SAN DIEGO, CA 92101-

Kód UT WoS kapitoly

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