Illuminating Enterovirus 71 RNA Replication Sites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00139133" target="_blank" >RIV/00216224:14740/24:00139133 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.jyu.fi/en/events/europic-2024" target="_blank" >https://www.jyu.fi/en/events/europic-2024</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Illuminating Enterovirus 71 RNA Replication Sites

Popis výsledku v původním jazyce

Enterovirus 71 (EV71), a member of the Picornaviridae family, poses a significant threat as a cause of hand-foot-and-mouth disease (HFMD), primarily impacting small children. The potential for severe neurological complications, leading to mortality rates of up to 19%, highlights the need for research efforts to address this pathogen1. With the escalating outbreaks in the Asia-Pacific region, the exploration of novel therapeutic approaches is indispensable. While current vaccines target specific serotypes, their efficacy lacks evidence of cross-protection. This underscores the importance of investigating targeted antiviral therapies1.2. However, progress is hindered by gaps in understanding EV71's replication cycle. This study aims to examine the unmapped facets of enterovirus RNA replication, focusing on its interaction with genome packaging and viral assembly. A key challenge in unravelling the molecular intricacies of the replication process is the development of a fluorescent reporter system to visualise viral replication sites. We present the establishment of a fluorescent reporter that enables specific labelling of EV71 replication sites without altering the viral genome, hence preserving the natural course of genome replication. This system will serve as a crucial tool for guiding subsequent research phases, including correlative light and electron microscopy for precise lamella preparation and cryo-electron tomography data collection.

Název v anglickém jazyce

Illuminating Enterovirus 71 RNA Replication Sites

Popis výsledku anglicky

Enterovirus 71 (EV71), a member of the Picornaviridae family, poses a significant threat as a cause of hand-foot-and-mouth disease (HFMD), primarily impacting small children. The potential for severe neurological complications, leading to mortality rates of up to 19%, highlights the need for research efforts to address this pathogen1. With the escalating outbreaks in the Asia-Pacific region, the exploration of novel therapeutic approaches is indispensable. While current vaccines target specific serotypes, their efficacy lacks evidence of cross-protection. This underscores the importance of investigating targeted antiviral therapies1.2. However, progress is hindered by gaps in understanding EV71's replication cycle. This study aims to examine the unmapped facets of enterovirus RNA replication, focusing on its interaction with genome packaging and viral assembly. A key challenge in unravelling the molecular intricacies of the replication process is the development of a fluorescent reporter system to visualise viral replication sites. We present the establishment of a fluorescent reporter that enables specific labelling of EV71 replication sites without altering the viral genome, hence preserving the natural course of genome replication. This system will serve as a crucial tool for guiding subsequent research phases, including correlative light and electron microscopy for precise lamella preparation and cryo-electron tomography data collection.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/EH22_008%2F0004607" target="_blank" >EH22_008/0004607: Nové technologie pro translační výzkum ve farmaceutických vědách /NETPHARM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů