AFM application across the life sciences field and its combination with special techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00142219" target="_blank" >RIV/00216224:14740/25:00142219 - isvavai.cz</a>

Výsledek na webu

<a href="https://afmbiomed.org/Barcelona-2025-scientific-program.aspx" target="_blank" >https://afmbiomed.org/Barcelona-2025-scientific-program.aspx</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

AFM application across the life sciences field and its combination with special techniques

Popis výsledku v původním jazyce

These approaches enhance the obtained data by integrating AFM (topography, mechanics) with information from other techniques within the same region of interest. It provides key insights into the structural and biomechanical behaviour of biological systems in their native state and physiological environments, enabling a deeper understanding of complex processes such as biomolecule imaging and cell mechanics. AFM’s versatility is further enhanced through its combination with other techniques, such as Raman spectroscopy, fluorescence microscopy, and scanning electron microscopy (SEM). While Raman spectroscopy provides precise chemical characterization, fluorescence microscopy enables the specific localization of desired interactions. Moreover, the correlative AFM-in-SEM enables precise in-situ data correlation, combining nanoscale topography with different information from SEM channels. Additionally, advanced AFM techniques, such as Fluid AFM, enable nanomanipulation, including single-cell nano-injection, expanding its applications in biomedical research. These combined methodologies provide a deeper understanding across a wide range of life sciences, including cellular processes, biomaterial interactions, and disease-related mechanical alterations, making AFM an excellent and useful tool for modern biological and biomedical research.

Název v anglickém jazyce

AFM application across the life sciences field and its combination with special techniques

Popis výsledku anglicky

These approaches enhance the obtained data by integrating AFM (topography, mechanics) with information from other techniques within the same region of interest. It provides key insights into the structural and biomechanical behaviour of biological systems in their native state and physiological environments, enabling a deeper understanding of complex processes such as biomolecule imaging and cell mechanics. AFM’s versatility is further enhanced through its combination with other techniques, such as Raman spectroscopy, fluorescence microscopy, and scanning electron microscopy (SEM). While Raman spectroscopy provides precise chemical characterization, fluorescence microscopy enables the specific localization of desired interactions. Moreover, the correlative AFM-in-SEM enables precise in-situ data correlation, combining nanoscale topography with different information from SEM channels. Additionally, advanced AFM techniques, such as Fluid AFM, enable nanomanipulation, including single-cell nano-injection, expanding its applications in biomedical research. These combined methodologies provide a deeper understanding across a wide range of life sciences, including cellular processes, biomaterial interactions, and disease-related mechanical alterations, making AFM an excellent and useful tool for modern biological and biomedical research.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů