Dual-Organoid Biosensor for Monitoring Cardiac Conduction Disturbances In Vitro
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143785" target="_blank" >RIV/00216224:14740/25:00143785 - isvavai.cz</a>
Výsledek na webu
<a href="http://nanometrologie.cz/cz/seminar.php" target="_blank" >http://nanometrologie.cz/cz/seminar.php</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Dual-Organoid Biosensor for Monitoring Cardiac Conduction Disturbances In Vitro
Popis výsledku v původním jazyce
Atomic Force Microscopy (AFM) is traditionally employed as a high-resolution imaging tool to analyze surface morphology and mechanical properties by scanning a sharp tip mounted on a flexible cantilever. Robust feedback system and micromechanical transducers are responsible for the constant adjustment of tip-sample interaction; however, it can be utilized to monitor the contraction dynamics of cardiomyocytes. Moreover, by distinguishing vertical and lateral contractile movements, AFM enables precise differentiation between focal and conductive arrhythmic contractions. This presentation introduces an AFM-based biosensor using a dual-beating human pluripotent stem cell (hPSC)-derived organoid. The high biosensor sensitivity allows detailed analysis of contractile behavior under pharmacological modulation with cardiomodulating drugs. The dual-organoid system enhances model robustness by minimizing variability inherent 19 in single-cell studies, thereby improving its translational relevance for cardiotoxicity assessment.
Název v anglickém jazyce
Dual-Organoid Biosensor for Monitoring Cardiac Conduction Disturbances In Vitro
Popis výsledku anglicky
Atomic Force Microscopy (AFM) is traditionally employed as a high-resolution imaging tool to analyze surface morphology and mechanical properties by scanning a sharp tip mounted on a flexible cantilever. Robust feedback system and micromechanical transducers are responsible for the constant adjustment of tip-sample interaction; however, it can be utilized to monitor the contraction dynamics of cardiomyocytes. Moreover, by distinguishing vertical and lateral contractile movements, AFM enables precise differentiation between focal and conductive arrhythmic contractions. This presentation introduces an AFM-based biosensor using a dual-beating human pluripotent stem cell (hPSC)-derived organoid. The high biosensor sensitivity allows detailed analysis of contractile behavior under pharmacological modulation with cardiomodulating drugs. The dual-organoid system enhances model robustness by minimizing variability inherent 19 in single-cell studies, thereby improving its translational relevance for cardiotoxicity assessment.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10403 - Physical chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů