Cerebrospinal fluid oligoclonal IgM test in routine practice: Comparison with quantitative assessment of intrathecal IgM synthesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A90128%2F20%3A00140072" target="_blank" >RIV/00216224:90128/20:00140072 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17110/20:A21026QG RIV/00843989:_____/20:E0108434

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0009898120302187?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0009898120302187?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cca.2020.05.021" target="_blank" >10.1016/j.cca.2020.05.021</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cerebrospinal fluid oligoclonal IgM test in routine practice: Comparison with quantitative assessment of intrathecal IgM synthesis

Popis výsledku v původním jazyce

Background: Intrathecal IgM synthesis demonstrated either as cerebrospinal fluid (CSF)-restricted oligoclonal (o-) IgM bands or calculated using various formulas has been linked to more aggressive multiple sclerosis (MS) course. However, the proportion of MS patients showing intrathecal IgM synthesis varies largely between studies. We aimed to explore the relation between different formulas and results of o-IgM, and to assess the frequency of o-IgM bands in an unselected series of samples. Methods: 432 samples were analyzed for o-IgM, o-IgG and quantitative measures of IgM and IgG synthesis. IgM index and formulas of Reiber, Auer and Ohman were compared to the result of the o-IgM test. Results: At the cut-off commonly used, the non-linear formulas for intrathecal synthesis were specific (&gt; 94%) but rather insensitive (&lt; 40% even at a cut-off of 4 CSF-restricted bands) compared to o-IgM. No significant difference was noted in the performance of different formulas. At a cut-off of 4 bands, 61% of MS patients, but none of the controls were positive for o-IgM. Conclusions: Formulas for intrathecal IgM synthesis are insensitive compared to o-IgM. We propose to evaluate samples with 2 or 3 extra-CSF IgM bands as borderline and only samples with 4 or more as definitely positive.

Název v anglickém jazyce

Cerebrospinal fluid oligoclonal IgM test in routine practice: Comparison with quantitative assessment of intrathecal IgM synthesis

Popis výsledku anglicky

Background: Intrathecal IgM synthesis demonstrated either as cerebrospinal fluid (CSF)-restricted oligoclonal (o-) IgM bands or calculated using various formulas has been linked to more aggressive multiple sclerosis (MS) course. However, the proportion of MS patients showing intrathecal IgM synthesis varies largely between studies. We aimed to explore the relation between different formulas and results of o-IgM, and to assess the frequency of o-IgM bands in an unselected series of samples. Methods: 432 samples were analyzed for o-IgM, o-IgG and quantitative measures of IgM and IgG synthesis. IgM index and formulas of Reiber, Auer and Ohman were compared to the result of the o-IgM test. Results: At the cut-off commonly used, the non-linear formulas for intrathecal synthesis were specific (&gt; 94%) but rather insensitive (&lt; 40% even at a cut-off of 4 CSF-restricted bands) compared to o-IgM. No significant difference was noted in the performance of different formulas. At a cut-off of 4 bands, 61% of MS patients, but none of the controls were positive for o-IgM. Conclusions: Formulas for intrathecal IgM synthesis are insensitive compared to o-IgM. We propose to evaluate samples with 2 or 3 extra-CSF IgM bands as borderline and only samples with 4 or more as definitely positive.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

30230 - Other clinical medicine subjects

Projekt

—

Návaznosti

—

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinica Chimica Acta

ISSN

0009-8981

e-ISSN

—

Svazek periodika

508

Číslo periodika v rámci svazku

NA

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

137-145

Kód UT WoS článku

000549228300021

EID výsledku v databázi Scopus

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