Kinetika hydrolýzy acetylthiocholinu pomocí cholinesteráz

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Kinetics of Hydrolysis of Acetylthiocholine by Cholinesterases.

Popis výsledku v původním jazyce

Alzheimer's disease (AD) is a chronic, incurable, neurodegenerative disorder. As one of the most common reason for AD lack of acetylcholine is considered. This deficit can be caused by too high concentration and/or activity of enzymes which breakdown acetylcholine. The most successful therapeutic strategy for AD tratment is application of cholinesterase inhibitors. The ideal goal of our research is to find a new potential drug against the Alzheimer´s disease. Acetylthiocholine (ATCH) is used for testingof enzymatic hydrolysis of acetylcholine (ACH) in vitro, because the mechanism of the hydrolysis is qualitatively similar to ACH and its reaction course can be quantitatively measured on-line by two independent methods: spectrophotometric (the Ellman´smethod; determination of product thiocholine) and electrochemical (pH-stat method; determination of the product acetic acid). We used the Ellman´s method for testing the mechanism of noninhibited enzymatic hydrolysis of ATCH by butyrylcho

Název v anglickém jazyce

Kinetics of Hydrolysis of Acetylthiocholine by Cholinesterases.

Popis výsledku anglicky

Alzheimer's disease (AD) is a chronic, incurable, neurodegenerative disorder. As one of the most common reason for AD lack of acetylcholine is considered. This deficit can be caused by too high concentration and/or activity of enzymes which breakdown acetylcholine. The most successful therapeutic strategy for AD tratment is application of cholinesterase inhibitors. The ideal goal of our research is to find a new potential drug against the Alzheimer´s disease. Acetylthiocholine (ATCH) is used for testingof enzymatic hydrolysis of acetylcholine (ACH) in vitro, because the mechanism of the hydrolysis is qualitatively similar to ACH and its reaction course can be quantitatively measured on-line by two independent methods: spectrophotometric (the Ellman´smethod; determination of product thiocholine) and electrochemical (pH-stat method; determination of the product acetic acid). We used the Ellman´s method for testing the mechanism of noninhibited enzymatic hydrolysis of ATCH by butyrylcho

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

European Congress of Young Chemists, Youngchem 2004

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

111

Název nakladatele

Warsaw University of Technology

Místo vydání

Varšava

Místo konání akce

Jurata, Polsko

Datum konání akce

13. 10. 2004

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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