Vliv S-adenosylmethioninu na funkci hepatocytů potkanů poškozených D-galaktosaminem

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine.

Popis výsledku v původním jazyce

Being a key intermediate in transmethylation and transsulphuration reactions, S-adenosyl-L-methionine (SAMe) exerts a possible hepatoprotective effect. We evaluated the ability of SAMe to improve the functions of rat hepatocytes damaged by D-galactosamine (GalN). Hepatocytes were isolated from Wistar rats (220 - 250 g) by collagenase perfusion and cultured in William´s E medium. After cell attachment GalN (40 mM) +/- SAMe (50 and 1000 mg/L) were added to media. Hepatocyte functional capacity and integrity were analysed by albumin secretion activity (ELISA kit, Bethyl Lab.), urea synthesis (kit, Sigma Aldrich) and lactate dehydrogenase (LDH) activity (kit, Sigma Aldrich) in cultivation media after 24 and 48 h incubation. Activities of mitochondrial dehydrogenases were determined by colorimetric assay based on the cleavage of the tetrazolium salt WST-1. GalN increased significantly LDH leakage from the cells during 24 h and 48 h incubation and decreased albumin secretion activity. SAMe s

Název v anglickém jazyce

The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine.

Popis výsledku anglicky

Being a key intermediate in transmethylation and transsulphuration reactions, S-adenosyl-L-methionine (SAMe) exerts a possible hepatoprotective effect. We evaluated the ability of SAMe to improve the functions of rat hepatocytes damaged by D-galactosamine (GalN). Hepatocytes were isolated from Wistar rats (220 - 250 g) by collagenase perfusion and cultured in William´s E medium. After cell attachment GalN (40 mM) +/- SAMe (50 and 1000 mg/L) were added to media. Hepatocyte functional capacity and integrity were analysed by albumin secretion activity (ELISA kit, Bethyl Lab.), urea synthesis (kit, Sigma Aldrich) and lactate dehydrogenase (LDH) activity (kit, Sigma Aldrich) in cultivation media after 24 and 48 h incubation. Activities of mitochondrial dehydrogenases were determined by colorimetric assay based on the cleavage of the tetrazolium salt WST-1. GalN increased significantly LDH leakage from the cells during 24 h and 48 h incubation and decreased albumin secretion activity. SAMe s

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clin. Chim. Acta suppl.

ISBN

0009-8981

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

152

Název nakladatele

Elsevier

Místo vydání

UK

Místo konání akce

Glasgow, Skotsko

Datum konání akce

8. 5. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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