Simultánní stanovení ubichinolu-10 a ubichinonu-10 v lidské plazmě pomocí HPLC s elektrochemickou detekcí

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The simultaneous determination of ubiquinol-10 and ubiquinone-10 in human plasma using HPLC with electrochemical detection

Popis výsledku v původním jazyce

Coenzyme Q10 is an important endogenous lipid-soluble antioxidant. Ubiquinol-10, the reduced form of coenzyme Q10 protects cells from oxidative damage. The oxidized form of coenzyme Q10 is known as ubiqinone-10. The ubiquinol-10/ ubiquinone-10 ratio maybe used as a marker for oxidative damage. The aim was to develop a HPLC method for the simultaneous determination of ubiquinol 10 and ubiquinone 10 in human plasma. Plasma for analysis was collected into vacutainer tubes containing EDTA. Tubes were centrifuged and extracted with hexane and methanol. Sample was injected onto 125 x 4 mm RP-C18 column (Purospher, 5 um; Merck). The mobile phase consisted of methanol-2-propanol-sodium acetate. We had filtered this mobile phase through a 0.45 um nylon filterand than deaerated before use. The flow rate was 1 ml/min and temperature was set to 37°C. We detected coenzyme Q10 with ESA Coulochem II 5100A electrochemical detector. After separation, the components passed the conditioning cell (-600

Název v anglickém jazyce

The simultaneous determination of ubiquinol-10 and ubiquinone-10 in human plasma using HPLC with electrochemical detection

Popis výsledku anglicky

Coenzyme Q10 is an important endogenous lipid-soluble antioxidant. Ubiquinol-10, the reduced form of coenzyme Q10 protects cells from oxidative damage. The oxidized form of coenzyme Q10 is known as ubiqinone-10. The ubiquinol-10/ ubiquinone-10 ratio maybe used as a marker for oxidative damage. The aim was to develop a HPLC method for the simultaneous determination of ubiquinol 10 and ubiquinone 10 in human plasma. Plasma for analysis was collected into vacutainer tubes containing EDTA. Tubes were centrifuged and extracted with hexane and methanol. Sample was injected onto 125 x 4 mm RP-C18 column (Purospher, 5 um; Merck). The mobile phase consisted of methanol-2-propanol-sodium acetate. We had filtered this mobile phase through a 0.45 um nylon filterand than deaerated before use. The flow rate was 1 ml/min and temperature was set to 37°C. We detected coenzyme Q10 with ESA Coulochem II 5100A electrochemical detector. After separation, the components passed the conditioning cell (-600

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clin. Chim. Acta suppl.

ISBN

0009-8981

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

227

Název nakladatele

Elsevier

Místo vydání

UK

Místo konání akce

Glasgow, Skotsko

Datum konání akce

8. 5. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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