Sledování antioxidačních vlastností kyseliny močové u lidí na základě měření hladin alantoinu v plazmě pomocí kapalinové chromatografie

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00002827" target="_blank" >RIV/00216275:25310/06:00002827 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography

Popis výsledku v původním jazyce

Uric acid is the end product of purine metabolism in humans. It has been pointed out, that uric acid acts as antioxidant and is capable to react with biologically relevant oxidants to form allantoin. Therefore, measurement of allantoin in humans was proposed as marker of oxidative stress. We estimated allantoin in human plasma obtained from the patients with chronic renal failure before hemodialysis (n = 30), the patients with non insulin dependent diabetes mellitus (n = 30) and blood donors (n = 30) using a method based on selective isolation of allantoin from deproteinized plasma samples on AG 1 X8 resin and its derivatization to glyoxylate 2,4 dinitrophenyl hydrazone. The method is free from urate and glyoxylate interferences. Separation of glyoxylate 2,4 dinitrophenyl hydrazone from other hydrazones was achieved on reversed phase HPLC with gradient elution and UV/VIS detection at 360 nm. The analytical performance of this method is satisfactory with intra assay CV 5.7%, inter assay

Název v anglickém jazyce

Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography

Popis výsledku anglicky

Uric acid is the end product of purine metabolism in humans. It has been pointed out, that uric acid acts as antioxidant and is capable to react with biologically relevant oxidants to form allantoin. Therefore, measurement of allantoin in humans was proposed as marker of oxidative stress. We estimated allantoin in human plasma obtained from the patients with chronic renal failure before hemodialysis (n = 30), the patients with non insulin dependent diabetes mellitus (n = 30) and blood donors (n = 30) using a method based on selective isolation of allantoin from deproteinized plasma samples on AG 1 X8 resin and its derivatization to glyoxylate 2,4 dinitrophenyl hydrazone. The method is free from urate and glyoxylate interferences. Separation of glyoxylate 2,4 dinitrophenyl hydrazone from other hydrazones was achieved on reversed phase HPLC with gradient elution and UV/VIS detection at 360 nm. The analytical performance of this method is satisfactory with intra assay CV 5.7%, inter assay

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinica Chimica Acta

ISSN

0009-8981

e-ISSN

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Svazek periodika

neuveden

Číslo periodika v rámci svazku

365

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

249-256

Kód UT WoS článku

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EID výsledku v databázi Scopus

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