Stanovení 4-hydroxynonenalu v biologickém materiálu pomocí vysokoúčinné kapalinové chromatografie

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of 4-hydroxynonenal in biological material by high-performance liquid chromatography

Popis výsledku v původním jazyce

Background. A result of free radical induced oxidative stress is lipid peroxidation. Many products are formed by peroxidation of lipids, for example hydrocarbons, alcohols, esters and carbonyl compounds. Among the most important carbonyl compounds that are formed by lipid peroxidation belongs malondialdehyde, formaldehyde, acetaldehyde, propionaldehyde, acetone, acrolein, and 4-hydroxynonenal. 4-Hydroxynonenal is the most significant aldehydic compound formed by peroxidation of omega-6-unsaturated fattyacids, it is very toxic and very reactive. It is easy determinable in biological samples even under physiological conditions. Methods. In this study we consider determination of 4-hydroxynonenal in biological material in order to use it as possible marker of oxidative stress and lipid peroxidation. For a derivatization of 4-hydroxynonenal we have used 1,3-cyclohexanedion. A fluorescent decahydroacridine derivate was determind by high-performance liquid chromatography with fluorescence d

Název v anglickém jazyce

Determination of 4-hydroxynonenal in biological material by high-performance liquid chromatography

Popis výsledku anglicky

Background. A result of free radical induced oxidative stress is lipid peroxidation. Many products are formed by peroxidation of lipids, for example hydrocarbons, alcohols, esters and carbonyl compounds. Among the most important carbonyl compounds that are formed by lipid peroxidation belongs malondialdehyde, formaldehyde, acetaldehyde, propionaldehyde, acetone, acrolein, and 4-hydroxynonenal. 4-Hydroxynonenal is the most significant aldehydic compound formed by peroxidation of omega-6-unsaturated fattyacids, it is very toxic and very reactive. It is easy determinable in biological samples even under physiological conditions. Methods. In this study we consider determination of 4-hydroxynonenal in biological material in order to use it as possible marker of oxidative stress and lipid peroxidation. For a derivatization of 4-hydroxynonenal we have used 1,3-cyclohexanedion. A fluorescent decahydroacridine derivate was determind by high-performance liquid chromatography with fluorescence d

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clinical Chemistry and Laboratory Medicine

ISBN

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ISSN

1434-6621

e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Walter de Gruyter GmbH & Co. KG

Místo vydání

Berlin

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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