HPLC stanovení 25-hydroxyvitamínu D3 v séru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00006367" target="_blank" >RIV/00216275:25310/07:00006367 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

HPLC assay for 25-hydroxyvitamin D3 in serum

Popis výsledku v původním jazyce

Vitamin D and its metabolites are important for the regulation of calcium homeostasis and for bone metabolism. The most important metabolites of vitamin D are 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. The determination of 25-hydroxyvitamin D3 was made by high-performance liquid chromatography with ultraviolet detection. For the separation, reverse phase column LiChroCart 125-4 Purospher RP-18e, 5 um, was used. The mixture of methanol and deionized water (95:5, v/v) was used as mobile phase. This method was applied to determination the concentration of 25-hydroxyvitamin D3 in a group of blood donors. The preliminary reference ranges of 25-hydroxyvitamin D3 in a group of blood donors are 10.42-116.20 nmol/L. The statistic analysis of the data measured was processed, including comparing the values obtained from the blood donors with supplementation of vitamin D and the ones without the supplementation of vitamin D. No statistically significant data diversity was found out in the

Název v anglickém jazyce

HPLC assay for 25-hydroxyvitamin D3 in serum

Popis výsledku anglicky

Vitamin D and its metabolites are important for the regulation of calcium homeostasis and for bone metabolism. The most important metabolites of vitamin D are 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. The determination of 25-hydroxyvitamin D3 was made by high-performance liquid chromatography with ultraviolet detection. For the separation, reverse phase column LiChroCart 125-4 Purospher RP-18e, 5 um, was used. The mixture of methanol and deionized water (95:5, v/v) was used as mobile phase. This method was applied to determination the concentration of 25-hydroxyvitamin D3 in a group of blood donors. The preliminary reference ranges of 25-hydroxyvitamin D3 in a group of blood donors are 10.42-116.20 nmol/L. The statistic analysis of the data measured was processed, including comparing the values obtained from the blood donors with supplementation of vitamin D and the ones without the supplementation of vitamin D. No statistically significant data diversity was found out in the

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Vitamins 2007 - Nutrition and Diagnostics

ISBN

978-80-7194-937-4

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

217

Název nakladatele

Univerzita Pardubice

Místo vydání

Pardubice

Místo konání akce

—

Datum konání akce

—

Typ akce podle státní příslušnosti

—

Kód UT WoS článku

—