Characterization of Fatty Acid and Triacylglycerol Composition in Animal Fats Using Silver-ion and Non-aqueous Reversed-phase High-performance Liquid Chromatography/Mass Spectrometry and Gas Chromatography/Flame Ionization Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892091" target="_blank" >RIV/00216275:25310/11:39892091 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/11:00367598

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2011.07.032" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2011.07.032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2011.07.032" target="_blank" >10.1016/j.chroma.2011.07.032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Fatty Acid and Triacylglycerol Composition in Animal Fats Using Silver-ion and Non-aqueous Reversed-phase High-performance Liquid Chromatography/Mass Spectrometry and Gas Chromatography/Flame Ionization Detection

Popis výsledku v původním jazyce

Non-aqueous reversed-phase and silver-ion high-performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection and GC/FID and mass spectrometry detection are used for the characterization of FA and TG composition in complex samples of animal fats from fallow deer, red deer, sheep, moufflon, wild boar, cock, duck and rabbit. The FA composition of samples is determined based on the GC/FID analysis of FA methyl esters. In total, 81 FAs of different acyl chain length, double bond COB) number, branched/linear, cis-/trans- and DB positional isomers are identified. TGs in animal fats contain mainly monounsaturated and saturated FAs. High amounts of branched and trans-FAs are observed in the samples of ruminants. InNARP mode, individual TG species are separated including the separation of trans- and branched TGs. Silver-ion mode provides the separation of TG regioisomers, which enables the determination of their ratios.

Název v anglickém jazyce

Characterization of Fatty Acid and Triacylglycerol Composition in Animal Fats Using Silver-ion and Non-aqueous Reversed-phase High-performance Liquid Chromatography/Mass Spectrometry and Gas Chromatography/Flame Ionization Detection

Popis výsledku anglicky

Non-aqueous reversed-phase and silver-ion high-performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection and GC/FID and mass spectrometry detection are used for the characterization of FA and TG composition in complex samples of animal fats from fallow deer, red deer, sheep, moufflon, wild boar, cock, duck and rabbit. The FA composition of samples is determined based on the GC/FID analysis of FA methyl esters. In total, 81 FAs of different acyl chain length, double bond COB) number, branched/linear, cis-/trans- and DB positional isomers are identified. TGs in animal fats contain mainly monounsaturated and saturated FAs. High amounts of branched and trans-FAs are observed in the samples of ruminants. InNARP mode, individual TG species are separated including the separation of trans- and branched TGs. Silver-ion mode provides the separation of TG regioisomers, which enables the determination of their ratios.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F09%2F0139" target="_blank" >GA203/09/0139: Komplexní lipidomická charakterizace rostlinných a živočišných tkání</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1218

Číslo periodika v rámci svazku

—

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

7499-7510

Kód UT WoS článku

000296037900010

EID výsledku v databázi Scopus

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