Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892094" target="_blank" >RIV/00216275:25310/11:39892094 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2011.05.081" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2011.05.081</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2011.05.081" target="_blank" >10.1016/j.chroma.2011.05.081</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry

Popis výsledku v původním jazyce

In this work, we describe a new method for the characterization of the lipid composition in various tissues, using off-line two-dimensional coupling of HILIC and RP-HPLC coupled to ESI and APCI mass spectrometry. In the first dimension the total lipid extracts are fractioned using HILIC into individual lipid classes. In total, 19 lipid classes (+3 regioisomeric pairs) that cover a wide range of polarities are separated in one analytical run, which is the highest number of analyzed lipid classes reportedso far. The lysophospholipid regioisomers are also separated in HILIC mode followed by the identification based on the characteristic ESI mass spectra. The collected fractions of the various lipid classes are further separated in the RP mode, which offers an excellent resolution of the individual lipid species. Their ESI or APCI mass spectra give correct information on the fatty acid composition and on the individual regioisomeric positions on the glycerol skeleton.

Název v anglickém jazyce

Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry

Popis výsledku anglicky

In this work, we describe a new method for the characterization of the lipid composition in various tissues, using off-line two-dimensional coupling of HILIC and RP-HPLC coupled to ESI and APCI mass spectrometry. In the first dimension the total lipid extracts are fractioned using HILIC into individual lipid classes. In total, 19 lipid classes (+3 regioisomeric pairs) that cover a wide range of polarities are separated in one analytical run, which is the highest number of analyzed lipid classes reportedso far. The lysophospholipid regioisomers are also separated in HILIC mode followed by the identification based on the characteristic ESI mass spectra. The collected fractions of the various lipid classes are further separated in the RP mode, which offers an excellent resolution of the individual lipid species. Their ESI or APCI mass spectra give correct information on the fatty acid composition and on the individual regioisomeric positions on the glycerol skeleton.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GP203%2F09%2FP249" target="_blank" >GP203/09/P249: Nové stacionární fáze pro separaci lipidů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1218

Číslo periodika v rámci svazku

—

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

5146-5156

Kód UT WoS článku

000293432200015

EID výsledku v databázi Scopus

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