Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892377" target="_blank" >RIV/00216275:25310/11:39892377 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >http://dx.doi.org/10.1007/s10815-011-9617-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >10.1007/s10815-011-9617-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid

Popis výsledku v původním jazyce

Purpose: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). Methods: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. Results: The cultured GCs were maintained for 45 days with a doubling time of 159+-24 hours. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10+-0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. Conclusion: Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.

Název v anglickém jazyce

Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid

Popis výsledku anglicky

Purpose: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). Methods: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. Results: The cultured GCs were maintained for 45 days with a doubling time of 159+-24 hours. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10+-0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. Conclusion: Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Assisted Reproduction and Genetics

ISSN

1058-0468

e-ISSN

—

Svazek periodika

28

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

939-950

Kód UT WoS článku

000296645200010

EID výsledku v databázi Scopus

—