Assessment of glutathione reductase inhibition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892670" target="_blank" >RIV/00216275:25310/11:39892670 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of glutathione reductase inhibition

Popis výsledku v původním jazyce

Glutathione reductase is a crucial enzyme for maintaining of intracellular glutathione levels. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to the reduced form (GSH). The aim of our study was to estimate a possible inhibitory effect on glutathione reductase activity in the presence of two substances that cause glutathione depletion - ethacrynic acid and diethyl maleate. We also tested glutathione as a possible inhibitor. GR activity was determined using spectrophotometricmethod based on measurement of absorbance decline (lambda = 340 nm) due to oxidation of NADPH. We found dose dependent inhibitory effect through reduced glutathione - the presence of 10 mM GSH caused a decrease in enzyme activity by 60%. We conclude that our finding of inhibitory effect in presence of glutathione is of great importance since the GSH levels are very high in the cells.

Název v anglickém jazyce

Assessment of glutathione reductase inhibition

Popis výsledku anglicky

Glutathione reductase is a crucial enzyme for maintaining of intracellular glutathione levels. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to the reduced form (GSH). The aim of our study was to estimate a possible inhibitory effect on glutathione reductase activity in the presence of two substances that cause glutathione depletion - ethacrynic acid and diethyl maleate. We also tested glutathione as a possible inhibitor. GR activity was determined using spectrophotometricmethod based on measurement of absorbance decline (lambda = 340 nm) due to oxidation of NADPH. We found dose dependent inhibitory effect through reduced glutathione - the presence of 10 mM GSH caused a decrease in enzyme activity by 60%. We conclude that our finding of inhibitory effect in presence of glutathione is of great importance since the GSH levels are very high in the cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

ISSN

1211-5541

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

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Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

77-83

Kód UT WoS článku

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EID výsledku v databázi Scopus

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