Asymmetric Flow Field Flow Fractionation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895141" target="_blank" >RIV/00216275:25310/12:39895141 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/9780470027318.a9289" target="_blank" >http://dx.doi.org/10.1002/9780470027318.a9289</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/9780470027318.a9289" target="_blank" >10.1002/9780470027318.a9289</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Asymmetric Flow Field Flow Fractionation

Popis výsledku v původním jazyce

The chapter provides overview of the theory and principles of asymmetric flow field flow fractionation (A4F). Effect of various operational parameters on the resolution of the A4F technique is discussed. For the sake of better understanding the A4F method is contrasted with widely known size exclusion chromatography (SEC). In contrast to SEC, in which the size restricted permeation of macromolecules into the pores of different size is the major separation mechanism, A4F separates the molecules without contribution of stationary phase. That is the most distinguished feature of the method that offers several advantages over SEC. The focus is on the combination of A4F with a multi-angle light scattering (MALS) detector that allows direct determination ofmolar mass and size distribution and characterization of branching. Theoretical and descriptive part is accomplished by several examples of experimental results obtained by the measurements of typical polymers, nanoparticles and proteins.

Název v anglickém jazyce

Asymmetric Flow Field Flow Fractionation

Popis výsledku anglicky

The chapter provides overview of the theory and principles of asymmetric flow field flow fractionation (A4F). Effect of various operational parameters on the resolution of the A4F technique is discussed. For the sake of better understanding the A4F method is contrasted with widely known size exclusion chromatography (SEC). In contrast to SEC, in which the size restricted permeation of macromolecules into the pores of different size is the major separation mechanism, A4F separates the molecules without contribution of stationary phase. That is the most distinguished feature of the method that offers several advantages over SEC. The focus is on the combination of A4F with a multi-angle light scattering (MALS) detector that allows direct determination ofmolar mass and size distribution and characterization of branching. Theoretical and descriptive part is accomplished by several examples of experimental results obtained by the measurements of typical polymers, nanoparticles and proteins.

Druh

C - Kapitola v odborné knize

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Encyclopedia of Analytical Chemistry

ISBN

978-0-470-02731-8

Počet stran výsledku

18

Strana od-do

1-18

Počet stran knihy

2351

Název nakladatele

John Wiley & Sons Ltd.

Místo vydání

Chichester

Kód UT WoS kapitoly

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