Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895677" target="_blank" >RIV/00216275:25310/12:39895677 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2012.10.041" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2012.10.041</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2012.10.041" target="_blank" >10.1016/j.chroma.2012.10.041</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography

Popis výsledku v původním jazyce

In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency forlow-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds,in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion m

Název v anglickém jazyce

Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography

Popis výsledku anglicky

In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency forlow-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds,in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion m

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Dvourozměrné separace v kapalných médiích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1268

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

91-101

Kód UT WoS článku

000312177300012

EID výsledku v databázi Scopus

—