Structural Characterization of Electrochemically and in Vitro Biologically Generated Oxidation Products of Atorvastatin Using UHPLC/MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896258" target="_blank" >RIV/00216275:25310/13:39896258 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/13:10190907

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s00216-013-7133-5" target="_blank" >http://link.springer.com/article/10.1007/s00216-013-7133-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-013-7133-5" target="_blank" >10.1007/s00216-013-7133-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural Characterization of Electrochemically and in Vitro Biologically Generated Oxidation Products of Atorvastatin Using UHPLC/MS/MS

Popis výsledku v původním jazyce

Ultrahigh-performance liquid chromatography coupled with high-mass-accuracy tandem mass spectrometry (UHPLC-MS-MS) has been used for elucidation of the structures of oxidation products of atorvastatin (AT), one of the most popular commercially availabledrugs. The purpose of the study was identification of AT metabolites in rat hepatocytes and comparison with electrochemically generated oxidation products. AT was incubated with rat hepatocytes for 24 h. Electrochemical oxidation of AT was performed by use of a three-electrode off-line system with a glassy carbon working electrode. Three supporting electrolytes (0.1 mol L-1 H2SO4, 0.1 mol L-1 HCl, and 0.1 mol L-1 NaCl) were tested, and dependence on pH was also investigated. AT undergoes oxidation by asingle irreversible process at approximately +1.0 V vs. Ag/AgCl electrode. The results obtained revealed a simple and relatively fast way of determining the type of oxidation and its position, on the basis of characteristic neutral losses

Název v anglickém jazyce

Structural Characterization of Electrochemically and in Vitro Biologically Generated Oxidation Products of Atorvastatin Using UHPLC/MS/MS

Popis výsledku anglicky

Ultrahigh-performance liquid chromatography coupled with high-mass-accuracy tandem mass spectrometry (UHPLC-MS-MS) has been used for elucidation of the structures of oxidation products of atorvastatin (AT), one of the most popular commercially availabledrugs. The purpose of the study was identification of AT metabolites in rat hepatocytes and comparison with electrochemically generated oxidation products. AT was incubated with rat hepatocytes for 24 h. Electrochemical oxidation of AT was performed by use of a three-electrode off-line system with a glassy carbon working electrode. Three supporting electrolytes (0.1 mol L-1 H2SO4, 0.1 mol L-1 HCl, and 0.1 mol L-1 NaCl) were tested, and dependence on pH was also investigated. AT undergoes oxidation by asingle irreversible process at approximately +1.0 V vs. Ag/AgCl electrode. The results obtained revealed a simple and relatively fast way of determining the type of oxidation and its position, on the basis of characteristic neutral losses

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

405

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

7181-7193

Kód UT WoS článku

000323653400004

EID výsledku v databázi Scopus

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