Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896727" target="_blank" >RIV/00216275:25310/13:39896727 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection

Popis výsledku v původním jazyce

A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine.

Název v anglickém jazyce

Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection

Popis výsledku anglicky

A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

ISSN

1211-5541

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

prosinec

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

25-38

Kód UT WoS článku

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EID výsledku v databázi Scopus

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