QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898080" target="_blank" >RIV/00216275:25310/14:39898080 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN

Popis výsledku v původním jazyce

Tau protein plays a crucial role in the neuronal cytoskeleton stabilization. Under the pathological conditions, it can be abnormally phosphorylated which leads to the aggregation and formation of neurofibrillary tangles representing pathological hallmarkof Alzheimer's disease (AD). For its association with neurodegenerative diseases, tau protein is intensively studied in various diagnostic and therapeutic applications. Since there is no standard of tau protein involving essential post-translational modifications, it is often necessary to purify it directly from cerebrospinal fluid (CSF) or blood of healthy or AD clinical signs exhibiting organism. The immunomagnetic purification based on the isolation of the target protein using a specific antibody bound to a magnetic carrier is the most effective tool for this purpose. High quality antibodies are the main prerequisite of successful purification, but many commercial antibodies do not comply with the challenging requirements for the im

Název v anglickém jazyce

QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN

Popis výsledku anglicky

Tau protein plays a crucial role in the neuronal cytoskeleton stabilization. Under the pathological conditions, it can be abnormally phosphorylated which leads to the aggregation and formation of neurofibrillary tangles representing pathological hallmarkof Alzheimer's disease (AD). For its association with neurodegenerative diseases, tau protein is intensively studied in various diagnostic and therapeutic applications. Since there is no standard of tau protein involving essential post-translational modifications, it is often necessary to purify it directly from cerebrospinal fluid (CSF) or blood of healthy or AD clinical signs exhibiting organism. The immunomagnetic purification based on the isolation of the target protein using a specific antibody bound to a magnetic carrier is the most effective tool for this purpose. High quality antibodies are the main prerequisite of successful purification, but many commercial antibodies do not comply with the challenging requirements for the im

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

ISSN

1211-5541

e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

září 2014

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

17

Strana od-do

147-163

Kód UT WoS článku

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EID výsledku v databázi Scopus

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