Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899345" target="_blank" >RIV/00216275:25310/14:39899345 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS

Popis výsledku v původním jazyce

Nevertheless, it is important to investigate these effects in more details for better understanding of lipids properties and for accurate quantitation. Methods Sets of PCs with fixed fatty acyl (14:0, 17:0 or 18:1) in sn-1 position and varied in length and saturation degree of fatty acyls in sn-2 position of glycerol backbone were synthesized. Samples with various concentrations of lipids were mixed with several MALDI matrices including DHB, 9-aminoacridine and diaminonaphthalene. Results MALDI-MS investigation of synthesized PC sets revealed the dependence of the intensity of lipid-related peaks on fatty acyl lengths. The character of the dependence was changed depending on used matrices and concentrations of lipids. In case of DHB matrix, the dependence was not monotonic. Peak intensities increased with the chain length increasing for samples with higher lipids concentrations and decreased for lower concentrations. The fact of lipid peaks decrease for longer fatty acyl chains for sam

Název v anglickém jazyce

Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS

Popis výsledku anglicky

Nevertheless, it is important to investigate these effects in more details for better understanding of lipids properties and for accurate quantitation. Methods Sets of PCs with fixed fatty acyl (14:0, 17:0 or 18:1) in sn-1 position and varied in length and saturation degree of fatty acyls in sn-2 position of glycerol backbone were synthesized. Samples with various concentrations of lipids were mixed with several MALDI matrices including DHB, 9-aminoacridine and diaminonaphthalene. Results MALDI-MS investigation of synthesized PC sets revealed the dependence of the intensity of lipid-related peaks on fatty acyl lengths. The character of the dependence was changed depending on used matrices and concentrations of lipids. In case of DHB matrix, the dependence was not monotonic. Peak intensities increased with the chain length increasing for samples with higher lipids concentrations and decreased for lower concentrations. The fact of lipid peaks decrease for longer fatty acyl chains for sam

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů