Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39898280" target="_blank" >RIV/00216275:25310/15:39898280 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081715:_____/15:00434034

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elps.201400413" target="_blank" >http://dx.doi.org/10.1002/elps.201400413</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201400413" target="_blank" >10.1002/elps.201400413</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

Popis výsledku v původním jazyce

In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol) diacrylate) cryogel placed in the centrifugal filter device. The composition of the polymerization mixture as well as the polymerization conditions wereoptimized in order to prepare a material with bimodal pore size distribution with 20-50 ?m flow through macropores and submicrometer pores in the polymer walls. The optimized, mechanically stable, highly porous, material was used for spin column lectin chromatography. The surface of the monolithic scaffold was activated by epichlorohydrin and used for immobilization of concanavalin A to provide the affinity supports for selective isolation of glycoproteins containing high mannose glycan structures. Theperformance of the developed lectin modified cryogels was evaluated by analyses of glycoprotein mixtures. The efficiency and selectivity of the affinity supports were confirmed by MALDI-MS analysis.

Název v anglickém jazyce

Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

Popis výsledku anglicky

In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol) diacrylate) cryogel placed in the centrifugal filter device. The composition of the polymerization mixture as well as the polymerization conditions wereoptimized in order to prepare a material with bimodal pore size distribution with 20-50 ?m flow through macropores and submicrometer pores in the polymer walls. The optimized, mechanically stable, highly porous, material was used for spin column lectin chromatography. The surface of the monolithic scaffold was activated by epichlorohydrin and used for immobilization of concanavalin A to provide the affinity supports for selective isolation of glycoproteins containing high mannose glycan structures. Theperformance of the developed lectin modified cryogels was evaluated by analyses of glycoprotein mixtures. The efficiency and selectivity of the affinity supports were confirmed by MALDI-MS analysis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-06319S" target="_blank" >GA14-06319S: Vývoj mikrofluidických rozhraní pro vícerozměrné separace v kapalné fázi s hmotnostní spektrometrií pro analýzy komplexních biologických vzorků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

11-12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

1344-1348

Kód UT WoS článku

000356004200014

EID výsledku v databázi Scopus

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